METHODS OF STAINING NERVOUS TISSUE

P-97: A Novel Staining Method of MouseOvarian Tissue for Comparison of Two Different Methods of Vitrification

Background: Different cryoprotectants are used for Cryopreservation of ovarian tissue in patients who are at risk of infertility. Ethylene glycol (EG), dimethyl sulfoxide (DMSO) and propanediol (PROH) were chosen as the basic permeable cryoprotectants due to their less glass-formation characteristic compared to the others. In the present study, the effects of two different vitrification methods...

The staining of degeneration in the nervous system of the octopus by modified silver methods

Introduction DEGENERATING axons of cephalopod material can be stained with a Cajal method (Sereni and Young, 1932). Whilst this has proved extremely valuable for demonstrating principal fibre connexions, it becomes more difficult to interpret the results when identifying degenerating fibres which are diffusely distributed within neuropil, or in tracts composed mainly of normal fibres. This is l...

Nervous Tissue

Two Step Cold Staining Methods

In developing countries pulmonary tuberculosis is usually diagnosed by detecting acid-fast bacilli (AFB) in sputum using a Ziehl-Neelsen (Z-N) staining method. However, in the field the traditional method of staining is difficult to carry out. This study evaluates the efficiency of two cold staining methods, namely Gabbet’s and a modified two reagent cold staining method compared with the Z-N t...

Non-nuclear staining of thick tissue sections.